Journal: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

Article Title: Serous tubal intraepithelial carcinoma upregulates markers associated with high-grade serous carcinomas including Rsf-1 (HBXAP), cyclin E and fatty acid synthase

doi: 10.1038/modpathol.2010.60

Figure Lengend Snippet: Immunoreactivity of p53, Ki-67, Rsf-1, cyclin E, FASN and mucin-4 in a STIC located in the fimbria (patient 11). Upper panel shows the STIC at low magnification. The epithelial cells at the right margin of the STIC show intense p53 expression but lack Ki-67 immunoreactivity (boxes). In the Middle and Lower panels the increased Rsf-1 and cyclin E immunoreactivity is confined to the same region (intense p53 expression but no Ki-67 immunoreactivity). In contrast, FASN is overexpressed only in the region where there is strong p53 expression and an elevated Ki-67 proliferation index. Mucin-4 immunoreactivity (lower panel) is largely absent in the STIC but positive in the normal tubal epithelium.

Article Snippet: The sources and dilution for each antibody were: Rsf-1 antibody (Millipore Upstate Cell Signaling, Billerica, MA; 1:2000 dilution), fatty acid synthase antibody (FASgen, Baltimore, MD; 1:50 dilution), cyclin E antibody (Zymed, Invitrogen; 1:250 dilution), mucin-4 antibody (Abcam, Cambridge, MA; 1:1500 dilution), p53 antibody (Ventana, Tucson, AZ; 1:1 dilution), Ki-67 antibody (Ventana, Tucson, AZ; 1:1 dilution) and p16 antibody (Cintec, Kirkland, WA; 1:1 dilution).

Techniques: Expressing

Journal: Alcohol and Alcoholism (Oxford, Oxfordshire)

Article Title: Effects of Moderate Ethanol Consumption on Lipid Metabolism and Inflammation Through Regulation of Gene Expression in Rats

doi: 10.1093/alcalc/agy079

Figure Lengend Snippet: Gene expression related to lipid metabolism and inflammation. The ethanol group showed significantly decreased hepatic expression of Hmgcr (P = 0.034), Srebp2 (P = 0.026), Cox-2 (P = 0.016) and RelA (P = 0.047) as well as significantly increased expression of and Pon-1 (P = 0.029) and Aldh2 (P = 0.002). *P ≤ 0.05 considered significant. Aldh2: aldehyde dehydrogenase-2; Cox2: cyclooxygenase-2; Hmgcr: 3-hydroxy-3-methylglutaryl-CoA reductase; Pon-1: paraoxonase-1; RelA: NF-kappa-B p65 subunit; RQ: relative quantification; Srebp2: sterol regulatory element-binding protein 2.

Article Snippet: The mRNA levels of 3-hydroxy-3-methylglutaryl-coenzyme A reductase ( Hmgcr , Rn00565598_m1), fatty acid synthase ( Fas , Rn00569117_m1), sterol regulatory element-binding protein-1 ( Srebp-1 , Rn01495769_m1), sterol regulatory element-binding protein-2 ( Srebp-2 , Rn01306296_m1), paraoxonase-1 ( Pon-1 , Rn01455909_m1), cyclooxygenase-2 ( Cox-2 , Rn00568225_m1), NF-kappa-B p65 subunit ( RelA , Rn01502266_m1) and aldehyde dehydrogenase-2 ( Aldh2 , Rn00583474_m1) were assessed by quantitative Real Time-PCR (ViiA7 system, Applied Biosystems, Foster City, CA) using TaqMan Universal PCR probes.

Techniques: Gene Expression, Expressing, Quantitative Proteomics, Binding Assay

Journal: Genes

Article Title: Chronic Mild Stress and Venlafaxine Treatment Were Associated with Altered Expression Level and Methylation Status of New Candidate Inflammatory Genes in PBMCs and Brain Structures of Wistar Rats

doi: 10.3390/genes12050667

Figure Lengend Snippet: The specification of primers used for the analysis of methylation levels in the promoter regions of the studied genes.

Article Snippet: TaqMan gene expression assay (Thermo Fisher Scientific, Waltham, Massachusetts, USA) was used to examine the expression of the following genes: IKBKB (assay ID: Rn00584379_m1), TGFA (assay ID: Rn00446234_m1), TGFB (assay ID: Rn00572010_m1), IRF1 (assay ID: Rn01483828_m1), PTGS2 (assay ID: Rn01483828_m1).

Techniques: Methylation, Sequencing

Journal: Genes

Article Title: Chronic Mild Stress and Venlafaxine Treatment Were Associated with Altered Expression Level and Methylation Status of New Candidate Inflammatory Genes in PBMCs and Brain Structures of Wistar Rats

doi: 10.3390/genes12050667

Figure Lengend Snippet: mRNA expression of TGFA ( A ), TGFB ( B ), PTGS2 ( C ), IRF1 ( D ) and IKBKB ( E ) in PBMCs of animals exposed to chronic mild stress (CMS) for two weeks (control, stressed) and in animals exposed to CMS for seven weeks and administered vehicle (1 mL/kg) or venlafaxine (10 mg/kg) for five weeks (stressed/saline, stressed/venlafaxine, control/venlafaxine). Relative gene expression levels were estimated using the 2−ΔCt (Ct gene–Ct 18S) method. Data represent means ± SD. N = 6; ** p < 0.01; *** p < 0.001 relative to control group; ### p < 0.001 relative to stressed/saline group.

Article Snippet: TaqMan gene expression assay (Thermo Fisher Scientific, Waltham, Massachusetts, USA) was used to examine the expression of the following genes: IKBKB (assay ID: Rn00584379_m1), TGFA (assay ID: Rn00446234_m1), TGFB (assay ID: Rn00572010_m1), IRF1 (assay ID: Rn01483828_m1), PTGS2 (assay ID: Rn01483828_m1).

Techniques: Expressing, Control, Saline, Gene Expression

Journal: Genes

Article Title: Chronic Mild Stress and Venlafaxine Treatment Were Associated with Altered Expression Level and Methylation Status of New Candidate Inflammatory Genes in PBMCs and Brain Structures of Wistar Rats

doi: 10.3390/genes12050667

Figure Lengend Snippet: mRNA expression of TGFA ( A ), IKBKB ( B ), TGFB ( C ), IRF1 ( D ) and PTGS2 ( E ) in the brain structures of animals exposed to chronic mild stress (CMS) for two weeks (control, stressed) and in animals exposed to CMS for seven weeks and administered vehicle (1 mL/kg) or venlafaxine (10 mg/kg) for five weeks (control/venlafaxine, stressed/saline, stressed/venlafaxine). Relative gene expression levels were estimated using a 2−ΔCt (Ctgene–Ct18S) method. Data represent means ± SD. N = 6. * p < 0.05; ** p < 0.01 relative to control group. # p < 0.05; ## p < 0.01; ### p < 0.001 relative to stressed/saline group. & p < 0.05; && p < 0.01 relative to stressed group.

Article Snippet: TaqMan gene expression assay (Thermo Fisher Scientific, Waltham, Massachusetts, USA) was used to examine the expression of the following genes: IKBKB (assay ID: Rn00584379_m1), TGFA (assay ID: Rn00446234_m1), TGFB (assay ID: Rn00572010_m1), IRF1 (assay ID: Rn01483828_m1), PTGS2 (assay ID: Rn01483828_m1).

Techniques: Expressing, Control, Saline, Gene Expression

Journal: Genes

Article Title: Chronic Mild Stress and Venlafaxine Treatment Were Associated with Altered Expression Level and Methylation Status of New Candidate Inflammatory Genes in PBMCs and Brain Structures of Wistar Rats

doi: 10.3390/genes12050667

Figure Lengend Snippet: Methylation levels of the TGFA ( A ), IRF1 ( B ), PTGS2 ( C ) and IKBKB ( D ) promoter in brain regions of animals exposed to chronic mild stress (CMS) for two weeks (control, stressed) and in animals exposed to CMS for seven weeks, including five-week administration of vehicle (1 mL/kg) or venlafaxine (10 mg/kg) (control/venlafaxine, stressed/saline, stressed/venlafaxine). Data represent means ± SD. N = 6. * p < 0.05, ** p < 0.01, *** p < 0.001 relative to control group. ## p < 0.01 ###; p < 0.001 relative to stressed/saline group.

Article Snippet: TaqMan gene expression assay (Thermo Fisher Scientific, Waltham, Massachusetts, USA) was used to examine the expression of the following genes: IKBKB (assay ID: Rn00584379_m1), TGFA (assay ID: Rn00446234_m1), TGFB (assay ID: Rn00572010_m1), IRF1 (assay ID: Rn01483828_m1), PTGS2 (assay ID: Rn01483828_m1).

Techniques: Methylation, Control, Saline